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rwpe 1 cells  (ATCC)


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    ATCC rwpe 1 cells
    Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 <t>in</t> <t>RWPE-1</t> cells elevated their invasive potential.
    Rwpe 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rwpe+1/pmc13101708-36-0-4?v=ATCC
    Average 99 stars, based on 676 article reviews
    rwpe 1 cells - by Bioz Stars, 2026-06
    99/100 stars

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    1) Product Images from "AAA ATPase TRIP13 is overexpressed in prostate cancer and promotes tumor progression"

    Article Title: AAA ATPase TRIP13 is overexpressed in prostate cancer and promotes tumor progression

    Journal: Translational Oncology

    doi: 10.1016/j.tranon.2026.102743

    Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 in RWPE-1 cells elevated their invasive potential.
    Figure Legend Snippet: Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 in RWPE-1 cells elevated their invasive potential.

    Techniques Used: Western Blot, Expressing, Proliferation Assay, Control, Invasion Assay, Staining, Over Expression



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    Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 <t>in</t> <t>RWPE-1</t> cells elevated their invasive potential.
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    Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 <t>in</t> <t>RWPE-1</t> cells elevated their invasive potential.
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    Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 <t>in</t> <t>RWPE-1</t> cells elevated their invasive potential.
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    Image Search Results


    Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 in RWPE-1 cells elevated their invasive potential.

    Journal: Translational Oncology

    Article Title: AAA ATPase TRIP13 is overexpressed in prostate cancer and promotes tumor progression

    doi: 10.1016/j.tranon.2026.102743

    Figure Lengend Snippet: Role of TRIP13 in PCa cell proliferation and invasion. (A) Immunoblot analysis showed the protein expression of TRIP13 in DU145 and PC3 PCa cells treated with either of two specific or independent TRIP13 siRNA duplexes. (B, C) Cell proliferation assay of DU145 and PC3 cells using either of two TRIP13 siRNA duplexes or a non-targeting siRNA control. (D, E) DU145 and PC3 cells were used in the Boyden chamber Matrigel invasion assay, in which TRIP13 was transiently knocked down using either of two independent TRIP13 siRNA duplexes. Non-targeted siRNA-treated cells served as controls. The invaded cells were stained, and the absorbance was read at 560 nm. The bar graphs are shown with ± SEM. (F) Boyden chamber Matrigel invasion assay showing that overexpression of P4HA1 in RWPE-1 cells elevated their invasive potential.

    Article Snippet: RWPE-1 cells obtained from ATCC were cultured in K-SFM containing bovine pituitary extract (BPE, 50 μg/ml) and epidermal growth factor (EGF, 5 ng/ml).

    Techniques: Western Blot, Expressing, Proliferation Assay, Control, Invasion Assay, Staining, Over Expression